Composite

Part:BBa_K2737010:Design

Designed by: Xinyu Teng   Group: iGEM18_ECUST   (2018-10-06)


Pfur3-lacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1237
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Different site of fur box in promoter may influence efficiency of promoter.


Source

Fur box is from genome of E.coli, lacI is from vector pET28a+ or biobrick.

References